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Molekyylibiologian entsyymit

Tärkeimmät työkalut nukleiinihappojen käsittelyyn ja analysointiin

Molekyylibiologian entsyymit ovat katalyyttisiä proteiineja, joita käytetään muokkaamaan, monistamaan, leikkaamaan ja liittämään nukleiinihappoja (DNA ja RNA). Ne ovat keskeisiä PCR:ssä, kloonauksessa, sekvensoinnissa ja genomianalyysissä.

Valikoimamme tarjoaa huolellisesti valittuja laadukkaita tuotteita, jotka on testattu vaativiin tutkimuskäyttöihin.

Tyypit

Polymeraasit

DNA-synteesi mallin avulla. Käytetään PCR:ssä, sekvensoinnissa ja monistamisessa. Taq, Pfu, Q5 polymeraasit saatavilla.

PCR Sekvensointi Korkea tarkkuus

Ligaasit

DNA-fragmenttien yhdistäminen. Välttämättömiä molekyylikloonauksessa ja vektorien rakentamisessa. T4 DNA-ligaasi, Taq-ligaasi saatavilla.

Kloonaus Ligaatio Vektorit

Restriktioentsyymit

Tarkka DNA:n leikkaus tunnistetuista sekvensseistä. Yli 200 entsyymiä saatavilla, mukaan lukien EcoRI, HindIII, BamHI.

Digestio Kloonaus RFLP

Fosfataasit

Dephosphorylaatio nukleiinihapoista. Käytetään vektorien valmisteluun kloonausta varten. CIAP-fosfataasi saatavilla.

Kloonaus Vektorit Dephosphorylaatio

Kinaasit

Fosforylaatio DNA:sta, RNA:sta tai proteiineista. T4-polynukleotidikinaasiradioaktiiviseen merkitsemiseen tai linkkerien ligaatioon.

Fosforylaatio Merkitseminen Linkkerit

Nukleaasit

Nukleiinihappojen hajotus. DNAasit, RNAasit, S1-nukleaasi rakenneanalyysiin ja sekvenssien poistoon.

Digestio RNA-analyysi Rakenne

Valitut tuotteet 54 tuotetta

DTT (Molecular Biology Grade)

CE131

DTT (Molecular Biology Grade)

5 g GeneOn

NAD (Molecular Biology Grade)

CE196

NAD (Molecular Biology Grade)

1 g GeneOn

NBT (Molecular Biology Grade)

CE209

NBT (Molecular Biology Grade)

1 g GeneOn

OORA00229-1L - Molecular Biology Grade UltraPure Water

OORA00229-1L

OORA00229-1L - Molecular Biology Grade UltraPure Water

1L Aviva Systems Biology

OORA00230-1L - Molecular Biology Grade UltraPure Water

OORA00230-1L

OORA00230-1L - Molecular Biology Grade UltraPure Water

1L Aviva Systems Biology

DMSO, Molecular Biology Grade

40470006-1

DMSO, Molecular Biology Grade

100 mL Bio-WORLD

EGTA, Molecular Biology Grade

40500028-2

EGTA, Molecular Biology Grade

50 g Bio-WORLD

BCIP (Molecular Biology Grade)

CE108

BCIP (Molecular Biology Grade)

250 mg GeneOn

DAPI (Molecular Biology Grade)

CE117

DAPI (Molecular Biology Grade)

5 mg GeneOn

Tris (Molecular Biology Grade)

CE237

Tris (Molecular Biology Grade)

500 g GeneOn

Tris (Molecular Biology Grade)

ST761-100g

Tris (Molecular Biology Grade)

100 g Beyotime Biotech Inc

Sucrose for molecular biology

27580

Sucrose for molecular biology

500 Gms Sisco Laboratories

CHAPS (Molecular Biology Grade)

CE114

CHAPS (Molecular Biology Grade)

1 g GeneOn

HEPES (Molecular Biology Grade)

CE171

HEPES (Molecular Biology Grade)

100 g GeneOn

Water (Molecular Biology Grade)

CE243

Water (Molecular Biology Grade)

500 ml GeneOn

Glycin (Molecular Biology Grade)

CE158

Glycin (Molecular Biology Grade)

1 kg GeneOn

OORA00218-100IU - DNA Ligase T4 Molecular Biology Grade

OORA00218-100IU

OORA00218-100IU - DNA Ligase T4 Molecular Biology Grade

100Units Aviva Systems Biology

Agarose, Molecular Biology Grade

40100164-1

Agarose, Molecular Biology Grade

25 g Bio-WORLD

Agarose (Molecular Biology Grade)

abx299715-100g

Agarose (Molecular Biology Grade)

100 µg Abbexa

Lysozyme (Molecular Biology Grade)

CE188

Lysozyme (Molecular Biology Grade)

1 g GeneOn

Lysozyme (Molecular Biology Grade)

CE189

Lysozyme (Molecular Biology Grade)

10 g GeneOn

Cesium Sulphate Molecular Biology

103550 10

Cesium Sulphate Molecular Biology

10 g Unisource Chemicals

Urea for molecular biology, 99.5%

21113

Urea for molecular biology, 99.5%

500 Gms Sisco Laboratories

OORA00218-100UNITS - DNA Ligase T4 Molecular Biology Grade

OORA00218-100UNITS

OORA00218-100UNITS - DNA Ligase T4 Molecular Biology Grade

100Units Aviva Systems Biology

Nile Red For Molecular Biology

114550 1

Nile Red For Molecular Biology

1 g Unisource Chemicals

Xylene for molecular biology, 99.5%

45122

Xylene for molecular biology, 99.5%

250 ml Sisco Laboratories

20X SSC - Molecular Biology Grade

IBS-BS011

20X SSC - Molecular Biology Grade

1L iNtRON Biotechnology Inc

20X SSP - Molecular Biology Grade

IBS-BS012

20X SSP - Molecular Biology Grade

1L iNtRON Biotechnology Inc

Acetone for molecular biology, 99.8%

27498

Acetone for molecular biology, 99.8%

500 ml Sisco Laboratories

Glycine for molecular biology, 99.5%

64072

Glycine for molecular biology, 99.5%

100 Gms Sisco Laboratories

20X SSPE - Molecular Biology Grade

IBS-BS013

20X SSPE - Molecular Biology Grade

1L iNtRON Biotechnology Inc

Tween 20, Molecular Biology Grade

T9100-010

Tween 20, Molecular Biology Grade

100ml GenDepot

OORA00219-v1 - DNA Ligase T4 Molecular Biology Grade (500units)

OORA00219-v1

OORA00219-v1 - DNA Ligase T4 Molecular Biology Grade (500units)

500units Aviva Systems Biology

Methanol for molecular biology, 99.5%

96446

Methanol for molecular biology, 99.5%

500 ml Sisco Laboratories

Carbinol for molecular biology, 99.5%

34883

Carbinol for molecular biology, 99.5%

500 ml Sisco Laboratories

100mL Molecular Biology Grade - PK6

46-000-CI

100mL Molecular Biology Grade - PK6

PK6 Scientific Laboratory Supplies

500mL Molecular Biology Grade - PK6

46-000-CV

500mL Molecular Biology Grade - PK6

PK6 Scientific Laboratory Supplies

Formamide for molecular biology, 99.5%

30349

Formamide for molecular biology, 99.5%

500 ml Sisco Laboratories

Imidazole for molecular biology, 99.5%

61510

Imidazole for molecular biology, 99.5%

25 Gms Sisco Laboratories

Boric Acid, Molecular Biology Grade

40200060-1

Boric Acid, Molecular Biology Grade

500 g Bio-WORLD

D(+)-Sucrose (Molecular Biology Grade)

CE224

D(+)-Sucrose (Molecular Biology Grade)

500 g GeneOn

STM Buffer - Molecular Biology Grade

IBS-BS016

STM Buffer - Molecular Biology Grade

1L iNtRON Biotechnology Inc

20xSSC, Molecular Biology Grade, pH7.0

TBS5033-1L

20xSSC, Molecular Biology Grade, pH7.0

1L Tribioscience

Chloroform for molecular biology, 99.8%

96764

Chloroform for molecular biology, 99.8%

100 ml Sisco Laboratories

Glycerol 87 % (Molecular Biology Grade)

CE154

Glycerol 87 % (Molecular Biology Grade)

1 l GeneOn

MOPS buffer (Molecular Biology Grade)

CE194

MOPS buffer (Molecular Biology Grade)

100 g GeneOn

Urea, suitable for molecular biology

GE1210

Urea, suitable for molecular biology

500g Glentham Life Sciences

Agarose, Molecular Biology Grade, 100g

PC0701-100g

Agarose, Molecular Biology Grade, 100g

each Vivantis

Agarose, Molecular Biology Grade, 1kg

PC0701-1kg

Agarose, Molecular Biology Grade, 1kg

each Vivantis

Agarose, Molecular Biology Grade, 500g

PC0701-500g

Agarose, Molecular Biology Grade, 500g

each Vivantis

Dimethylsulfoxide (Molecular Biology Grade)

CE120

Dimethylsulfoxide (Molecular Biology Grade)

100 ml GeneOn

TritonX-100 (Molecular Biology Grade)

CE240

TritonX-100 (Molecular Biology Grade)

500 ml GeneOn

STET Solution - Molecular Biology Grade

IBS-BS014

STET Solution - Molecular Biology Grade

500 mL iNtRON Biotechnology Inc

General Molecular Biology Grade Water

NWT-020-01

General Molecular Biology Grade Water

500 mL Novatein Biosciences, Inc.

Tutkimussovellukset

Lisätietoa

Wikipedia - Entsyymit

Science Direct - Entsyymit

PubMed - Molekyylibiologian entsyymit

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