Golgi Staining Kit – Green Fluorescence | Cytopainter (ab139483) contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. Micromolar concentrations of the green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line Jurkat, and human bone osteosarcoma epithelial cell line, U2OS.
One important application of ab139483 is in fluorescence co-localization imaging with a red fluorescent protein (RFP)- or orange fluorescent protein (OFP)-tagged proteins, a powerful approach for determining the targeting of molecules to intracellular compartments and for screening of their associations and interactions.
Ab139483 is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.
- Product name: Golgi Staining Kit – Green Fluorescence – Cytopainter
- Sample type: Adherent cells, Suspension cells
- Assay type: Cell-based
- Species reactivity Reacts with: Mammals, Other species
Notes: This kit can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes and various fluorescent proteins in multi-color imaging and detection applications. The dye emits in the FITC region of the visible light spectrum and is resistant to photo-bleaching, concentration quenching, and photoconversion.