PCR of SARS-CoV-2 Variant SA/BR

CE IVD kit for detection of SARS-CoV-2 virus RNA and identification of its British, South African and Brazilian variants in one reaction.

gb SARS-CoV-2 Variant SA/BR, UK kit enables multiplex detection of SARS-CoV-2 virus RNA (Wuhan coronavirus 2019) in RdRP gene and simultaneously the detection of mutations A570D a E484K in S gene.

Validated for:

  1. CFX96/96Touch (Bio-Rad)
  2. QuantStudio 5 (Applied Biosystems)
  3. RG 3000 (Corbett Research)

The A570D mutation occurs exclusively in lineage B.1.1.7 (a.k.a 20I/501Y.V1) referred to as the British variant. The E484K is an escape mutation that causes reduced binding of neutralizing antibodies and occurs in lineage B.1.351 (a.k.a 501Y.V2) referred to as the South African variant and lineage P.1 (a.k.a 501Y.V3) referred to as the Brazilian variant. The false negativity is checked by exogenous positive control with artificial sequence.

The test is based on multiplex one-step RT-qPCR method using fluorescently labelled probes (virus detection in FAM, HEX, ROX channels) and exogenous positive control (Cy5 channel). More information about this product (Cat. no. 3239-100, Cat. no. 3239-500) and its user manual are available.

Gentaur Next day delivery prices:

gb SARS-CoV-2 Variant SA/BR, UK, 100 rxn 3239-100 683,00 EUR
gb SARS-CoV-2 Variant SA/BR, UK, 500 rxn 3239-500 3200,00 EUR

Other qPCR products


The best available CE IVD one-step RT-qPCR kit contains reagents to provide 100/500 reactions (20 μl volume of each reaction).


gb SARS-CoV-2 Influenza A/B


Content of the diagnostic kit

Cat. No. Product rxn

  1. 3233-100 gb SARS-CoV-2 Influenza A/B 100
  2. 3233-500 gb SARS-CoV-2 Influenza A/B 500

Validated for cyclers

Bio-Rad Opus

  1. Life Technologies – Applied Biosystems: real-time PCR instruments
  2. Life Technologies – Applied Biosystems: StepOne & StepOnePlus real-time PCR instruments
  3. Life Technologies – Applied Biosystems: 7500 FAST real-time PCR system
  4. Life Technologies – Applied Biosystems: ViiA 7
  5. Life Technologies – Applied Biosystems: OpenArray
  6. Life Technologies – Applied Biosystems: QuantStudio
  7. Roche: LightCycler 2.0
  8. Roche: LightCycler 96
  9. Roche: LightCycler 480 HRM in LC480
  10. Roche: LightCycler 1536
  11. Roche: LightCycler Nano
  12. Idaho Technology: Lightscanner32
  13. Idaho Technology: RapidCycler 2
  14. Idaho Technology: LightCycler
  15. Eppendorf: ep realplex
  16. Bio-Rad – Nucleic Acid Amplification and Detection systems
  17. Bio-Rad: CFX96 Touch & CFX384 Touch
  18. Bio-Rad: CFX Connect
  19. BioRad: iQ5 & MyiQ Cycler
  20. Bio-Rad: Chromo 4 & Opticon & Opticon2 & MiniOpticon
  21. Bio Molecular Systems: MIC qPCR
  22. Qiagen: Rotor-Gene Q HRM in Rotor-Gene Q
  23. Corbett Life Science: Rotor-Gene 6000
  24. Cepheid: SmartCycler, GeneXpert (close system only NatTrol)
  25. Agilent Technologies: Mx3000 & Mx3005P
  26. Stratagene: Mx3000P & Mx3005P
  27. Sacace: SaCycler – 96
  28. TAKARA: TP800
  29. Primerdesign: genesig q16
  30. Illumina: Eco qPCR
  31. Analytik Jena: qTOWER
  32. Thermo Scientific: PikoReal real-time PCR system
  33. Fluidigm: Biomark
  34. WaferGen SmartChip System
  35. Biotrove: OpenArray NT cycler
  36. BioGene: InSyte & SynChron Thermal Cycler
  37. DNA Technology: DT-322
  38. Exicycler
  39. TL998 System
  40. Bioer Technology
  41. Notebookthermal cycler
  42. Link -> Biocompare – real-time PCR platforms
  43. Bio-Rad – Nucleic Acid Amplification and Detection systems

Comparative Specifications forSars-Cov2 Real-Time PCR Covid Detection Systems

  1. Feature CFX Connect
  2. CFX Connect CFX96 Touch
  3. CFX96 Touch CFX384 Touch
  4. CFX384 Touch
  • Excitation range 450–535 nm 450–684 nm 450–650 nm
  • Light source 3 LEDs in optics shuttle 6 LEDs in optics shuttle 5 LEDs in optics shuttle
  • Optical detection 3 photodiodes 6 photodiodes 5 photodiodes
  • Detection range 515–580 nm 515–730 nm 515–690 nm
  • Multiplex capability Up to 2 targets Up to 5 targets Up to 4 targets
  • Gradient range 30–100°C 30–100°C 30–100°C
  • Maximum gradient span 24°C 24°C 24°C
  • Maximum ramp rate 5°C/sec 5°C/sec 2.5°C/sec


Dimensions


(W x D x H) 33 x 46 x 36 cm 33 x 46 x 36 cm 33 x 46 x 36 cm
Weight 21 kg 21 kg 21 kg
Base thermal cycler CFX Connect C1000 Touch C1000 Touch
DOWNLOAD — Bio-Rad real-time PCR Application guide
The CFX96 Touch™ System – Designed for the Way You Work

The CFX96 Touch real-time PCR detection system builds on the power and flexibility of the C1000 Touch thermal cycler to create an exceptional real-time PCR system. Its unsurpassed thermal cycler performance plus innovative optical design produce accurate, reliable data. The powerful yet intuitive CFX Manager software accelerates every step of your real-time PCR research, shortening the time between getting started and obtaining great results.

Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen
Get results right away – quick installation and factory calibrated optics let you set up the system in seconds
Fit experiments into your schedule – fast thermal cycling produces results in less than 30 minutes
Save research time – thermal gradient feature lets you optimize reactions in a single experiment
Minimize sample and reagent usage – perform up to five-target multiplexing using small sample volumes
Rely on performance – innovative technology with long-lasting LEDs and solid-state components provides maximum reliability and optimal quantitative results
Analyze results when and where you want – receive email notification with an attached data file when a run is finished
Configure the system to fit your laboratory needs – run without a computer, run up to four instruments from one computer, or integrate with the CFX automation system for higher throughput
Run high resolution melt (HRM) experiments on the CFX96 Touch or CFX384 Touch system and perform data analysis using seamless data import to Precision Melt Analysis™ software


More Information

With the CFX96 system, you can:
Get great results right away — quick installation and factory-calibrated optics let you set up the system in seconds
Fit experiments into your schedule — fast thermal cycling produces results in <30 minutes
Save research time — thermal gradient feature lets you optimize reactions in a single experiment
Minimize sample and reagent usage — reliable results obtained with sample volumes as low as 10 μl
Rely on performance — innovative technologies with long-lasting LEDs and solid-state components provide maximal reliability and optimal quantitative results
Analyze results when and where you want — software can send e-mail notification with attached data file when the run is finished
Easily expand your throughput when you need to — up to 4 instruments can be controlled by a single computer

Six-Channel Optics Shuttle

The CFX96 system’s solid-state optical technology (six filtered LEDs, each with a corresponding filtered photodiode) maximizes fluorescence detection for specific dyes in specific channels, providing sensitive detection for precise quantitation and target discrimination. Scanning just above the sample plate, the optics shuttle individually illuminates and reads fluorescence from each well with high sensitivity and no cross talk. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always optimal and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference.


CFX384 Touch™ Real-Time PCR Detection System

The CFX384 Touch real-time PCR detection system builds on the power and flexibility of the C1000 Touch™ thermal cycler to create a high-throughput real-time PCR system. With unrivaled stand-alone functionality, innovative optical design, and unsurpassed thermal cycler performance, the CFX384 Touch shortens the time between getting started and obtaining great results.


Features include:

qPCR that stands alone – quickly set up runs and monitor amplification traces in real time on the large integrated LCD touch screen
Generate robust results right away – quick installation and factory calibrated optics let you set up the system in seconds
Rely on performance – innovative technology with long-lasting LEDs and solid-state components provides maximum reliability and optimal quantitative results
Minimize sample and reagent usage – 4-target multiplex experiments yield optimal results using sample volumes as low as 3 μl
Analyze results when and where you want – receive email notification with an attached data file when a run is finished
Save research time – thermal gradient feature lets you optimize reactions in a single experiment
Configure the system to fit your laboratory needs – run without a computer, run up to four instruments from one computer, or integrate with the CFX automation system for higher throughput
Trust your results – confirm system performance and data reliability with internal qualification, internal reference spots, system test software, and IQ/OQ. Integrate the CFX384 real-time PCR detection system with good laboratory practice standards for data collection and analysis with CFX Manager™ software, Security Edition
More Information

CFX Connect™ Real-Time PCR Detection System

The CFX Connect real-time PCR detection system incorporates industry-leading optics with a Peltier-based reaction block for precise and reliable qPCR data acquisition. The system offers an integrated solution for all your SYBR®Green/EvaGreen and duplex experiments. A thermal gradient is easily generated across the 96-well reaction block to support rapid optimization of real-time PCR assays. The CFX Connect system includes the powerful CFX Manager softwarefor system operation, experiment setup, and data analysis.
Features include:

Easy startup – obtain great results right away with factory calibration, quick setup, and intuitive software
Effortless optimization – save time and reduce costs optimizing assays in a single run using the thermal gradient
Powerful data analysis tools – quickly and accurately validate and analyze your data with the advance analysis modules of CFX Manager software
Accelerate publication submission – include MIQE annotations and generate RDML files using Biogazelle’s qbasePLUS software
Run high resolution melt (HRM) experiments on the CFX Connect and perform data analysis using seamless data import to Precision Melt Analysis software
More Information

MyiQ Thermal Cycler

An Easy-to-Use Real-Time PCR Instrument From the Experts in Quantitative PCR

The new MyiQ real-time PCR detection system offers an affordable alternative for the detection of common green fluorescent dyes such as FAM and SYBR Green I. This system interfaces directly with the iCycler thermal cycler, offering superior features such as thermal gradient and Peltier-effect driven performance. The MyiQ real-time PCR detection system is a perfect solution for those just getting started with this technology as well as those looking for additional instruments to handle increasing routine assay demands. This system was developed by the same experts at Bio-Rad who have pioneered performance in real-time PCR. The MyiQ delivers the same excellent-quality data as the iCycler iQ real-time detection system.

The iQ5 real-time PCR detection system

The iQ5 real-time PCR detection system offers a fresh design for Bio-Rad’s new generation of real-time PCR instrumentation. Like its predecessors, the iCycler iQ and MyiQ systems, the iQ5 optical system is a modular upgrade to the iCycler thermal cycler with 96-well reaction block. This powerful new system offers five-target analysis capabilities for multiplex PCR, coupled with a completely redesigned software package.
The iQ5 optical system software offers a full suite of enhanced data collection and data analysis options, including tools for gene expression analysis that measure relative quantities without a standard curve. Regardless of your PCR application, you will find that the iQ5 real-time PCR detection system is the ultimate system for value and flexibility.

Key Features

Sars-Cov-2 Multiplexing of up to 5 fluorophores in each reaction vessel conserves precious samples and reagents
Real-time PCR thermal gradient enables simple, rapid assay optimization
Intuitive software for protocol setup and data analysis shortens time to results
Diverse data analysis and presentation options provide a comprehensive view of your assay results
Advanced gene expression analysis methods with publication-quality statistics and graphs for relative quantitation studies
Flexible allelic discrimination module allows easy-to-use, automatic sample identification in genotyping experiments
Embedded tools for end-point fluorescence analysis enables simple qualitative analysis of sample abundance in single- or multicolor assays
Robust, reliable thermal cycling performance delivered by the iCycler thermal cycler
Chromo4™ Four-Color Real-Time Detector (Bio-Rad)

The Chromo4 unit is a real-time detector that can be added to a DNA Engine® cycler
as readily as any of our interchangeable sample blocks.

The MJ Research® Chromo4 detector easily converts any DNA Engine thermal cycler into a real-time system, by simply swapping with any of our user-changeable sample blocks. The small size and flexibility of this instrument allow it to keep pace with the evolving needs of the laboratory. Product Specifications
Key Features:
Interchangeable detector fits standard DNA Engine base units, new or existing.
The Chromo4 detector slides into the base in just seconds without the need for tools.
Swappable Chromo4 photonics shuttle can be ordered with customized filter sets.
Multiplex with up to four colors. All 96 wells are independently excited and detected for each color to minimize crosstalk.
Compact size and broad dynamic range make this one of the smallest, most sensitive real-time systems.
DNA Engine thermal cycler offers precise thermal control and a temperature gradient feature.
Opticon 2 – Continuous Fluorescence Detection System
Multicolor capability allows detection of SYBR® Green I and FAM in the first channel, and a range of fluorophores in the second channel — including TET, HEX, VIC, and TAMRA — for a multitude of applications such as RT-qPCR and allelic discrimination

Product Specifications


DNA Engine® thermal cycler offers precision thermal control and a temperature gradient feature permits simultaneous incubation at 12 different temperatures to optimize reactions in a single run.
Real-time results allow for plotting of signal intensity vs. cycle number and graphically monitoring the thermal profile during the run.
Extraordinary sensitivity permits reliable detection of one initial template copy, while delivering a linear range of up to ten orders of magnitude in starting copy number (with appropriate chemistry).
Researcher-oriented software allows quantification of samples and generation of melting curves to verify product identity.
Innovative optical system incorporates an array of 96 LEDs for excitation and a pair of sensitive PMTs for detection in a robust, no-moving-part design.
High sample capacity accommodates up to 96 samples in standard, low-profile microplates or strip tubes making specialized disposables unnecessary.
A compact footprint (34cm wide x 47cm deep x 60cm high) ensures that the Opticon system comfortably fits on any lab bench.

The MiniOpticon uses illumination technology similar to that of the widely used Opticon™ 2 system—samples are sequentially illuminated by a fixed array of 48 blue-green light-emitting diodes (LEDs). Each LED beam is precisely focused onto its corresponding well to ensure minimal crosstalk and light scattering. Crosstalk is further reduced by sequentially illuminating and detecting each well. LEDs provide a long-lived, maintenance-free excitation source, for years of uninterrupted performance. Emitted fluorescence is detected by one of two filtered, sensitive photodiodes. This innovative no-moving-parts design allows accurate detection in a compact and robust package for real-time PCR applications and gel-free DNA analysis.


Purpose of use


gb SARS-CoV-2 Influenza A/B CE IVD kit is based on onestep RT-qPCR using fluorescently labelled probes enabling a
multiplex detection of viral genes of SARS-CoV-2, Influenza
A and Influenza B and exogenous control. The detection
method is based on officially recommended WHO and Institut


Pasteur designs for detection of SARS-CoV-2 and on usage
of our proprietary GEMINI™ probe technology.
Principle of detection
The test is based on multiplex one-step RT-qPCR method.
The analysis is performed using fluorescently labelled
probes, which allow the detection of viral genes SARS-CoV-2
(E, RdRP genes – FAM channel), Influenza A (HEX channel), and
Influenza B (ROX channel) and exogenous positive control
(Cy5 channel). The kit contains all the necessary components
to perform the test.
Parameters of the diagnostic kit

  1. • CE IVD multiplex one-step RT-qPCR kit
  2. • SARS-CoV-2 – detection of viral genes E and RdRP
  3. • Influenza A, Influenza B – detection of viral gene M
  4. • fluorescence channels FAM (SARS-CoV-2), HEX (Infl A),
  5. ROX (Infl B), Cy5 (EPC)
  6. • technology of GEMINI™ probes for high detection
  7. sensitivity
  8. • suitable for multi-channel PCR thermocyclers
  9. • distinguishing between Influenza A and Influenza B
  10. • LOD of kit is 2.15 cop/rxn for SARS-CoV-2, 14.68 cop/
  11. rxn for Influenza A and 10.67 cop/rxn for Influenza B
  12. (95% CI)
  13. • positive and negative control for analysis validity
  14. confirmation
  15. • exogenous control (EPC Template RNA) is an artificial
  16. RNA, which may be added either to the sample before
  17. the isolation or to the RT-qPCR reaction
  18. • exogenous control serves for verification of extraction
  19. process and reveals possible inhibition of RT-qPCR
  • Component 1) Volume Qty 2) Conc.
    Assay CoV-2 Influenza 0.5 ml 3) 1 | 5 4x
    Master Mix OneStep Multi 1.0 ml 3) 1 | 5 2x
    Positive Control CoV-2 0.2 ml 1 | 1 4x
    Positive Control Influenza A 0.2 ml 1 | 1 4x
    Positive Control Influenza B 0.2 ml 1 | 1 4x
    EPC Template RNA 1.0 ml 1 | 5
    Deionized Water 1.0 ml 1 | 1
    1) tube lid colour corresponds to reagent type
    2) number for kit size of 100 / 500 reactions
    3) volume equates to 100 PCR reactions of 20 µl volume