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Rat Leptin pegylated super antagonist (mutant D23L/L39A/D40A/F41A) Recombinant Protein
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Rat Leptin pegylated super antagonist (mutant D23L/L39A/D40A/F41A) Recombinant Protein

Arvostelut

€567.38* Preço aproximado

  • Luokka:

    Liiketoiminta ja teollisuus > Tutkimus- ja laboratoriotuotteet

  • Tuotenro: 209-500-056S
  • Koko: 50 µg
  • Toimittaja: Gentaur
Mono-pegylated (with 20 kDa PEG) recombinant super rat leptin antagonist is one polypeptide chain containing 146 amino, an additional Ala at N-terminus and one molecule of PEG 20 kDa at its N-terminus, having an expected molecular mass of ~ 35.6 kDa as determined by MS. However due to enlarged hydrodymanic volume human pegylated leptin antagonist runs on the SDS-PAGE as 48 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. Its half-life in circulation after SC injection was over 20 hours. Super rat leptin antagonist (SRLA) was initially mutated, resulting in D23L/L39A/D40A/F41A mutant that was purified by proprietary chromatographic techniques. Pegylation of human leptin antagonist similar to that described in Elinav et al. Endocrinology 150:3083-91 (2009) for PEG-MLA. More details can be found in Shpilman at al., J. Biol. Chem. 286:4439-42 (2011).

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