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NF-κB Reporter (Luc) - HCT116 Cell Line

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  • Luokka:

    Liiketoiminta ja teollisuus > Tutkimus- ja laboratoriotuotteet

  • Tuotenro: 421-60623
  • Koko: 2 vials
  • Toimittaja: Gentaur
NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The<br />firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of<br />the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors<br />bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling<br />in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated<br />molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern<br />(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for<br />establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.<br />This cell line can be further modified to allow investigation of downstream NF-κB activities as a<br />result of targeted genetic mutation(s).

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